Comparison of Cryopreservation of Mouse Sperm vs Embryos
|Advantage||Cheaper to cryopreserve, requires only one day of work, requires only two males/line||Embryos are frozen post fertilization, recovery to live offspring easier|
|Disadvantage||Only freeze one gamete, revival through IVF (labor extensive and costly), must use wildtype females as egg donors||Requires at least 8 females|
|# animals needed||2 males/line||One heterozygous or homozygous breeder male and 8-16 weanling females|
|# days to complete||One day||One to two days|
- Embryo cryopreservation is an efficient means of long-term storage of valuable strains.
- Embryo cryopreservation works well for double and triple gene mutations on standard genetic backgrounds.
- This service is available for both mice and rats.
- Cheaper and easier recovery to live offspring.
- Yearly storage fee depending on # samples stored.
Mouse embryo cryopreservation has the following steps:
- Contact Animal Models Core (firstname.lastname@example.org) to discuss scheduling.
- Assessm of animal colony to assure one proven male and at least 8 weaned females are available for superovulation. GEAM may need close to 15 females to obtain good quality embryos for cryopreservation.
- GEAM will perform IVF and cryopreserve two-cell embryos.
- We recommend freezing 100-125 embryos if homozygous or 175-200 heterozygous embryos are preserved.
- Recovery of live animals by thawing and surgically transferring the frozen embryos into pseudopregnant recipients.
|Mouse Embryo Cryopreservation||UW Price|
|1-5 donor females||$1010|
|6-10 donor females||$1160|
|11-14 donor females||$1240|