Comparison of Cryopreservation of Mouse Sperm vs Embryos
|Advantage||Cheaper to cryopreserve, requires only one day of work, requires only two males/line||Embryos are frozen post fertilization, recovery to live offspring easier|
|Disadvantage||Only freeze one gamete, revival through IVF (labor extensive and costly), must use wildtype females as egg donors||Requires at least 8 females|
|# animals needed||2 males/line||One heterozygous or homozygous breeder male and 8-16 weanling females|
|# days to complete||One day||One to two days|
- Sperm cryopreservation is an inexpensive and efficient means of long-term storage of valuable lines.
- Sperm cryopreservation works well for single gene mutations on standard genetic backgrounds.
- The only disadvantage is that live offspring must be recovered through in vitro fertilization (IVF).
Mouse sperm cryopreservation has the following steps:
- Contact the Animal Models Core (firstname.lastname@example.org) to discuss scheduling.
- Assess animal colony to assure two proven male breeders (per line) would be available.
- Mice are transferred to the Biotechnology center on day of freezing. GEAM will submit animal transfer form to coordinate transfer of males on the day of cryopreservation.
- GEAM will sacrifice males and access sperm motility and concentration and give report to labs post-cryo.
- Sperm will be frozen and stored in liquid nitrogen tanks within GEAM.
- Recovery of live animals occurs by thawing and performing an IVF then surgically transferring the fertilized embryos into pseudopregnant recipients.
|MOUSE SPERM CRYOPRESERVATION (RECOMMEND FREEZING TWO MALES/LINE)||UW PRICE|
|RAT SPERM CRYOPRESERVATION (RECOMMEND FREEZING TWO MALES/LINE)||UW PRICE|