Sperm Cryopreservation

Comparison of Cryopreservation of Mouse Sperm vs Embryos

Sperm Embryos
Advantage Cheaper to cryopreserve, requires only one day of work, requires only two males/line Embryos are frozen post fertilization, recovery to live offspring easier
Disadvantage Only freeze one gamete, revival through IVF (labor extensive and costly), must use wildtype females as egg donors Requires at least 8 females
# animals needed 2 males/line One heterozygous or homozygous breeder male and 8-16 weanling females
# days to complete One day One to two days
Cost $605/2 males $880-$1700

 

 

 

  • Sperm cryopreservation is an inexpensive and efficient means of long-term storage of valuable lines.
  • Sperm cryopreservation works well for single gene mutations on standard genetic backgrounds.
  • The only disadvantage is that live offspring must be recovered through in vitro fertilization (IVF).

Mouse sperm cryopreservation has the following steps:

  1. Contact the Animal Models Core (animalmodels@biotech.wisc.edu) to discuss scheduling.
  2. Assess animal colony to assure two proven male breeders (per line) would be available.
  3. Mice are transferred to the Biotechnology center on day of freezing. GEAM will submit animal transfer form to coordinate transfer of males on the day of cryopreservation.
  4. GEAM will sacrifice males and access sperm motility and concentration and give report to labs post-cryo.
  5. Sperm will be frozen and stored in liquid nitrogen tanks within GEAM.
  6. Recovery of live animals occurs by thawing and performing an IVF then surgically transferring the fertilized embryos into pseudopregnant recipients.
MOUSE SPERM CRYOPRESERVATION (RECOMMEND FREEZING TWO MALES/LINE) UW PRICE EXTERNAL PRICE
1 male/day $410 $635.50
2 males/day $605 $937.75
4 males/day $990 $1534.50
8 males/day $1800 $2790
RAT SPERM CRYOPRESERVATION (RECOMMEND FREEZING TWO MALES/LINE) UW PRICE EXTERNAL PRICE
1 male/day $700 $1085
2 males/day $825 $1278.75