The basic steps in a rederivation project are:
- Generation of embryos by mating superovulated females to males or generate timed pregnant moms
- Collection of embryos from females or perform c-sections
- Transfer of aseptic embryos or pups into the Level 1 females located in the Biotechnology Center
For projects involving the rederivation of pathogen-carrying strains from within the university, these animals will be transferred to GEAM quarantine facility in MSC for superovulation. For projects involving the transfer of a strain from another university, the investigator will need to arrange the transfer of these animals into GEAM quarantine facility in MSC.
We will superovulate and dissect reproductive tracts from the animals. We then collect the embryos from the reproductive tracts and transfer them into pseudopregant B6D2F1 (for mice) and SD (for rats) recipients. The number of transfers completed depends on the number of good quality embryos collected.
After pups are weaned recipient moms will be screened for pathogen status by serological testing. The pups will then be transferred to the investigator as soon as serology results indicate the recipient mom is pathogen-free and the animal transfer form has been completed and approved.
For more planning info, please see the following PDF:
RARC Rodent Import 2022